Composite

Part:BBa_K3722010:Design

Designed by: Yongzhu Li   Group: iGEM21_NWU-CHINA-A   (2021-09-30)


T7_Promoter-RBS-Fre-Linker-SttH-T7_Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2384
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1017
    Illegal NgoMIV site found at 1275
    Illegal AgeI site found at 1504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 480


Design Notes

The linker may affect substrate diffusion to the active site pocket or the flexibility of such linkers may reduce activity. Fre was effective as a soluble N-terminal tag for SttH.


Source

Aseembly

References